BIOCH410 Lecture Notes - Lecture 11: Proteasome Inhibitor, Cyclin-Dependent Kinase 1, Ubiquitin
12 Feb 2020
School
Department
Course
Professor
Document Summary
Model 1: cyclins act stoichiometrically as highly potent inhibitors. Cyclin: responsible for removing anti-mpf subunit in the prempf form of this conjugated o release the cdc2. The cdc2 and the mpf becomes active. Wee kinase realists in hyper-phosphorylated state of the complex (add more phosphate residues). leads to inhibition of the complex. Cdc25 phosphatases remove hyper-phosphorylation to reactivate the cyc-cdk complex. Very important for cell to have control mechanism at checkpoints. Restriction point: after this there is no way back. Make sure the cell is truly committed for a new stage of the cell cycle. It is important to do cdk inactivation to complete the cycle, to pass through the m phase. Anaphase telophase required ubiquitination of the cdk (for degradation) Met always incorporated to newly synthesized protein. At the end radio labelled met can further be studied in terms of mechanisms of its degradation. Full length in wt cyclin and truncated samples based on degradation.
