BIOCH200 Lecture Notes - Lecture 5: Flavin Mononucleotide, Oligonucleotide, Cell Nucleus
16 Sep 2016
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Biochem 200 - lecture #5 - nucleic acids. The structure of nucleic acids: by convention, nucleotide sequences are written from 5" (left) to 3" (right, agtc. Primary structure: overall charge of a nucleic acid. 1 per nucleotide (but depends on how many phosphates are attached: both dna and rna are polar. Rna is more polar in backbone, because of 2" oh. Can be written as atg or dadtdg. Hydrolysis of phosphodiester bonds: occurs in rna, spontaneous, occurs in alkaline conditions (ph > 10) If it occurs in acid, no cleavage occurs. Cytosine deaminates to form uracil: spontaneous reaction converting c to u, uncorrected in dna = cause mutation, one role for t vs u in dna. Dna repair mechanisms will recognize u and remove it. Hence more stable and important to maintain integrity. Properties of the bases: heterocyclic, aromatic, e- delocalization, basically planar, poorly soluble in h2o, largely hydrophobic with some polar groups (h-bonds, absorb light in uv range.
