MBB 201 Lecture Notes - Lecture 1: Central Dogma Of Molecular Biology, Bright-Field Microscopy, Macromolecule
Document Summary
Cells- small, membrane enclosed units filled with a concentrated aqueous solution of chemicals endowed with the extraordinary ability to create copies of themselves by growing and then dividing in two: fundamental units of life. All present-day cells have apparently evolved from the same ancestral cell: when a cell divides it creates a daughter cell and passes on its genetic information. If the information is corrupted a mutation occurs and the dna is changed. Preparing samples for microscopy: prepare thin sections, fixation- process of preserving the cell and preventing degradation, staining- adds colour for contrast and can bind to particular molecules in the cell. Light microscopes allow examination of cells and some of their components: the compound microscope is commonly used, uses multiple lenses. It is difficult to see the components of the cell because they have no colour so they are often stained.