BISC 101 Lecture Notes - Lecture 4: Restriction Enzyme, Phosphodiester Bond, Leucine
15 Dec 2017
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Dna is transcribed into mrna and in the nucleus and is sent out to be translated into protein in the cytoplasm. Bold under lined letter g marks the transcription start point. Bold underlined letters att marks the termination sequence. Top strand is the template strand, bottom is the coding strand. Write the sequence for rna strand, from 5" to 3". Translate the mrna from 5" to 3" and write down the amino acids of the resulting polypeptide. 5" methionine (start) leucine alanine cysteine - stop 3". Name two different methods for making many copies of a sequence of dna. There are 2 methods of gene cloning: in vivo, and in vitro (pcr). In vitro (polymerase chain reaction) can take small fragments of dna, heat to separate the two strands, and use taq polymerase to build new strands. It continues separating the strands and adding a complimentary strand, thus amplifying it exponentially.
