BIOL 102 Lecture Notes - Lecture 22: Polymerase Chain Reaction, Crispr, Genome Editing
Document Summary
Crispr is genome editing technology that can cheaply and easily modify genomes. Make use of a bacterial immune system that can cut a bacterial genome and insert viral dna. Can be used to cut eukaryotic dna @ speci c sites; introducing random mutations that disrupt a gene or insert novel dna. Spacers in bacteriophage dna are signatures of past potential bacterial pathogens. Bacterial genome have clustered regularly interspersed short palindromic repeats (crispr) Can add gene it never had before or replace a mutated gene. Synthetic guide rna is produced with end that matches gene to be targeted. Cas protein is protein associated with crispr. Cas + grna add to cell, grna binds to target gene and cas cuts dna. Producing many copies of a speci c dna segment. Need to use dna polymerase that would survive in a hot temp. Taq polymerase from a bacteria and was used in a 90+ temp (which is needed to separate the dna)
