BCHM 310 Lecture Notes - Lecture 24: Myoglobin, Dissociation Constant, Partial Pressure
3 Nov 2017
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Hemoglobin has to be ef cient in picking up o from the lungs and dropping it off at the site of the tissue. Partial pressure of oxygen: pretty good ? (00:53) Binding oxygen ef ciently in the lungs; release oxygen ef ciently in tissue. ** in this case, simplistic reversible binding is not suf cient. If you have a weak binding molecule, you won"t get (3:22) This is why simplistic binding just doesn"t work. This is where you have allosteric oxygen binding : positive cooperativity. We are going to be talking about hemoglobin but the methodology works for any cooperative system. The n value is going to change as you titrate more of the oxygen. In saturated amounts the pln complex is pretty standard. At subsaturated amounts, the concentration of ligand --- this is a complex set of species. Because partway through subsaturated amounts, the sites are going to be partially occupied bc oxygen is coming off and on.
