BIOL 301 Lecture Notes - Lecture 7: Single-Nucleotide Polymorphism, Taqman, Bioinformatics
9 May 2020
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Document Summary
Snps, chips and next gen dna sequencing. The disciplines of genomics and bioinformatics promise powerful new approaches to evolutionary genetics in the 21st century. Sequencing by synthesis: single nucleotide polymorphisms (snps) Most widespread in the genome and most valuable for potential genetic information. Taqman assays are one use of snps. Quick to carry out and are cheap to reproduce once developed. Taqman assays use pcr and fluorescence: microarrays. Allows expression of thousands of genes in. Allows expression of thousands of genes in different tissues to be tested simultaneously: massively parallel dna sequencing. Sequencing by synthesis involves taking a single strand of dna and then synthesizing its complement by enzyme activity. Different platforms for this have emerged, including illumina, 454, ion torrent and solid. Can acquire a wealth of data for one price. Can be used to sequence whole genomes. "you don"t need a ferrari to go grocery shopping"
