BIOLOGY 3P03 Lecture Notes - Lecture 3: Ouabain, Atp Hydrolysis, Dinitrophenol
20 Sep 2017
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Suggests that there is no net movement of potassium and a sign change occurs. V = voltage drop in volts: c = ka/d. D = distance separating the plates: biological membranes have high capacitance (c) due to small d" of the lipid bilayer; Lipid has a high k, whose value is relatively constant and easy to remember. Cmembrane = 1 f/cm2 or 1 pf/100 m2. (in reality, this value is closer to 0. 7 f/cm2: thus, we can calculate the amount of charge separated across the membrane capacitance for a voltage drop of, for e. g. , -58 mv. Answer: 5. 8 10-8 coulombs/cm2; determined using 1 cm2 cell membrane, which would have a capacitance of 10-6 f. Number of ions determined by dividing 5. 8 10-8 coulombs/cm2 by faraday"s constant (i. e. 105) to achieve 5. 8 10-13; then, multiply by avogadro"s number (i. e. 6. 022 1023) Note: coulombs/faraday"s constant = number of mols; number of mols .
