BIOLOGY 2EE3 Lecture Notes - Lecture 2: Horizontal Gene Transfer, Mutation, Metagenomics
12 Aug 2016
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Document Summary
Exploits the fact that polymerase needs 3" hydroxyl. Faster, cheaper, detects addition of nucleo at end of dna via light. Skips cloning straight to frag many rxns at once. Analysis of large data sets of seq data. Annotation help to id reading frame (start & stop of gene) Speed and cost incredible only raw ingo. Collection of cloned dna frag that represent genome. # number of cloned frag needed to encompass whole genome. P = probability f = avg size of frag / total genome. 2d page gel (isoelectronic point & mass) mass spec. Ortholog (same ancestor with same gene function, diff org) Areas of higher or lower g/c content are less xferable. Studying these areas provides insight into comparative genomics. Dna segments of 10-200kb associated with trna, transpossible elements, plasmids and bacteriophages. Construction of gene libraries from dna from microbial community. Helps to find archae, can be used for biotech or nanotech. Acquire sample seq analysis (eliminate known genes)
