BIOCHEM 3G03 Lecture Notes - Lecture 3: Phosphodiester Bond, Complementary Dna, Polynucleotide
4 Nov 2018
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Document Summary
Nucleotides has a nitrogen-containing base adenine, guanine = purines cytosine, thymine = pyrimidines. Nucleic acid structure linkage between nucleotides = phosphodiester bond bond formed between 5th carbon and 3rd carbon of next nucleotide. Dna molecules linked by hydrogen bonds (n-h, o-h) A pairs with t and c with g major features of a dna molecule: two polynucleotide strands are antiparallel - their phosphodiester bonds run in opposite directions one in 5"-3" other 3"-5" Nucleic acids denatured and renatured more conformational freedom than dna because isn"t constrained by base-pairing can old back on itself so base pairs form complementary segments of same strand. G-c pair stacking is stronger than a-t base pairs - so g-c rich helix harder to disrupt than a and t this is due to melting temperature of dna (tm) Dna denatures by increasing temperature and creating a curve - midpoint of curve = tm. Dna renatures on its own by forming h-bonds when temperature lowered.
