PHGY 209 Lecture Notes - Lecture 7: The Terminal, Ultracentrifuge, Blood Proteins
15 Apr 2018
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Document Summary
Differential precipitation by salts- precipitate proteins from plasma. Electrophoretic mobility- aa can dissociate at the acidic or basic radical and at a specific ph of plasma, the proteins can dissociate. There are various ways of separating these proteins on the basis of charge: electrophoresis- fractionation method based on movement of charged particles along a voltage gradient. Rate of migration is influenced by the number and distribution of charges and by the mw of each protein. Each protein migrates at its own characteristic rate the stains reveal protein bonds. There are sub classes of these plasma proteins. If we scan this band, we end up with a series of peaks. In renal disease, there is less albumin than typical because the kidneys give a lot of the albumin in the urine. In bacterial infection, a lot of antibodies are produced and so more gamma globulins.
