PHGY 209 Lecture Notes - Lecture 3: Vasopressin, Osmotic Concentration, Elastic Fiber
15 Apr 2018
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It could be a radioactive substance etc. this is called q. stir the mixture till the indicator is well mixed. Remove an aliquot (small measured volume) and measure the dilution of the indicator. The concentration is equaled to the total amount added divided by the concentration of aliquot. The plasma will be in communication with the isf etc. allow time to equilibrate. Remove known value of blood and centrifuge to obtain plasma. Measure concentration of plasma and calculate the v=q/c. If you want to measure totally body water. Inject indicator that should cross the layers of capillary wall isf and cell membrane- antipyrine is lipid soluble which means it can cross the cell membrane. If 50 ml of d2o has been injected and distributed equally. At equilibrium, a mm of plasma was taken, and . 001 ml d20 was found: depending on what part of the blood needs to be determined, pick an indicator that will only pass through so much.
