MIMM 211 Lecture Notes - Lecture 10: Chemostat, Hyperthermophile, Psychrophile

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Concentration of one of the nutrient should be provided in limiting amounts to reduce growth rate. All nutrients should be provided continuously at the same rate to the growing culture. Some of the growing culture (cells and waste products) should be removed continuously at the same rate. Growth rate is then constant and equals loss rate, same amounts of cells actively growing. Provide a constant supply of cells in balanced growth (exponential phase) and growing at a known growth rate. Study microbial growth at variable and very low nutrient concentrations, resemble the environment bacteria encounter in nature. Facilitate research in microbial interaction and ecological situations. Allow industrial production of food and metabolites continuously. Temperature (optimal growth temperature: hyperthermophiles (95, thermophiles (65, mesophiles (37, psychotrophs (25, psychrophiles (10) Hydrogen ion concentration (ph: neutrophiles (7, acidophiles (below 7, alkalophiles (above 7) Gaseous environment: aerobes (need o2, anaerobes (absence of o2, facultative (with/without o2, capnophiles (need oxygen and high co2 concentration)

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