MIMM 211 Lecture Notes - Lecture 15: Horizontal Gene Transfer, Pilus, Recbcd

• School: McGill University
• Department: Microbiology & Immunology
• Course: MIMM 211
• Professor: Benoit Cousineau

Document Summary

Homologous recomb: recbcd enzymes have helicase/nuclease capabilities. Snips and digests 5" end to create overhangs: reca facilitates the crossing over, filling in missing nucleotides, resolvase splices them back into two homologous dsdnas. Non reciprocal homologous recomb: one way street. Host and donor dna: donor dna is inserted and replaces host dna, get new info, way to get new dna sequences from a donor. Horizontal gene transfer: btwn different cells (vs vertical, which is passed down to daughter cells: conjugation: via sex pilus, cell-cell, transformation: uptake plasmid via environment, transduction: virus/bacteriophage inserted. Jump into chromosome to be replicated, then jump out again, based on equilibrium. Col plasmids (colicins, bacteriocins, natural antibiotic against secretors" competitors) Exogenote + endogenote = merozygote cell 4 fates of newly acquired dna: Partial diploid clone: plasmid can replicate, so all cells in resulting colony gets the same plasmid. Partial diploid cell: non replicating plasmid, so only that cell will have it.