MIMM 211 Lecture Notes - Lecture 9: Gut Flora, Chemostat, Doubling Time
18 Oct 2016
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Lec 9: growth curve, generation time, lag phase, chemostat. Lag phase exponential (log) phase stationary phase death phase. Lag: require to revv engine , make starting materials (ex. ribosomes) and replicate dna. Longer lag if cells (inoculum) are inoculated from: old culture in death phase, dormant state, rich poor medium. No lag when poor rich, will readjust by down-regulating genes that synthesize enzymes previously needed in lacking medium. Ex. switch carbon source from glucose to galactose, cell needs to discard glucose proteins and synthesize galactose metabolizers: chem different medium, cold (fridge) hot, hot cold. Exp: when environment is not limiting, adequate nutrients: lowest doubling time, max growth speed. Stationary: transition to stationary when cells consume enough nutrients and produce toxic metabolic products: we take cells in stationary phase for inoculation, env not very favourable, so ribosomes and dna are not replicated.
