BIOC 300D2 Lecture Notes - Lecture 6: Intron, Restriction Enzyme, Exonuclease
8 Jul 2016
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Document Summary
Polymerase chain reaction: a technique for amplification of genetic sequences. Principle: template dna containing the target sequence to be amplified is subjected to successive cycles of temperature changes. In addition to target dna, reaction mix contains. Two specific primers are annealed to the start and end points of target sequence. Note: 5" ends of specific primers denote start and end points. Two copies of dna are created which extend beyond the start and end points. *primer design: make sure that design them such that polymerase extends primers 5" to 3" Template denaturation: 4 ss dna molecules => primer annealing => primer extension. At the end of second cycle, no ds molecules of appropriate length has been produced. At the end of the 3rd cycle, the first pair of ds dna molecules with the length of the target sequence have been generated. These short products will be geometrically amplified in the following cycles.
