ANAT 565 Lecture Notes - Lecture 8: Proteasome, Lysine, Myc
3 Sep 2016
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Document Summary
Addition of 16c to cysteine residue on target. Mutate cysteine to serine ko pat function. Palmitoylation through dhhc motif lipid molecule anchors soluble proteins to membrane. Ras: small soluble g protein in survival, proliferation, adhesion, polarization. When encounters pat at golgi palmitoylated ras to pm, does function thioesterase cleave palmitoylation off ras ras back to golgi, cycle. Length of tm domain assist in localizing diff integral membrane proteins to diff membrane domains. Palm within tmd: can modify length of tm domain. Can tilt tm domain, move around membrane and then remove so set up at appropriate site later. Enable protein-protein interaction: allow tail come closer to another protein tm domain. Block protein-protein interaction: move cytosolic tail away from a soluble protein. Palmitoylation adjacent to tm: localize protein to lipid raft. Palmitic acid is molecule incorporated into protein, make radioactive to detect. Treat w/tritiated palmitic acid cell will take up & use as regular palmitate chain.
