ANAT 416 Lecture Notes - Lecture 12: Adult Stem Cell, Aldehyde Dehydrogenase, Flow Cytometry
3 Jun 2020
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Feb 16 // lec 12 // stem cells 2 // l mccaffrey. Things we look for in a stem cell: Can regenerate all cell types within its tissue. Ex. aldehyde dehydrogenase 1 converts something that is not fluorescent, aldefluor, to a fluorescent product in stem cells. Stem cells often have multi-drug resistant pumps to protect themselves from toxic substances. You can use fluorescent dyes-the stem cell pumps it out. There is a direct correlation between stemness and dye efflux. Non-sc take up the dye, it intercalates with dna, lights up the cell. Flow cytometry to look for the dark or side population (the population that excludes the dye). Flow cytometry: there is a machine that sucks up cells one at a time. As the cells pass through the machine, there is a laser that shoots a beam. You can couple lasers in order to determine what markers are present or absent from the cell.
