ANAT 262 Lecture Notes - Lecture 19: Epidermal Growth Factor Receptor, Ldl Receptor, Transmission Electron Microscopy

• School: McGill University
• Department: Anatomy & Cell Biology
• Course: ANAT 262
• Professor: Ursula Stochaj

Document Summary

Membrane receptors for lipoproteins accumulated in clathrin-coated pits, pit is endocytosed endocytic vesicles. Clathrin pits at cell surface, see mostly clathrin (rather than accessory proteins) Freeze fracture, fracture across pm, transmission electron microscope (clathrin also come from golgi) Heavy chains & light chains, heavy chains for structure. Core of hydrophobic lipids (triglycerides & cholesterol esters) Apob has large hydrophobic areas lipoprotein particles built around, binds to ldl receptor. Synthesized in hepatocytes, go through secretory pathway into blood. Capable of binding to ldl receptors on surface of many cells. Ap-2 found on cell surface, doesn"t require small gtpase. Ap-2 doesn"t bind to arf, binds at pm. Uncoating for ap-2 is slightly different than for other aps. Families had very high ldls in bloodstream, develop heart disease & die of heart attack early . Bind ldls but don"t concentrate in clathrin-coated pits inefficient endocytosis. Lead to discovery of tyrosine & dilucine motifs.