BIOL 300 Lecture Notes - Leucine Zipper, Cell Membrane, Adherens Junction
15 Dec 2012
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Document Summary
Scaffolds, as we said, have two main functions: pathway separation and speeding up pathways. We will continue to discuss the mating mechanism in yeast, mediated by 3 kinases binding to a scaffold protein. In this system is a positive regulator (ste50) which further enhances the pathway"s activity, and a negative regulator which inhibits it. They then further tinkered with this system by changing the types of leucine zippers they used as recruiters for. Ste50, with different levels of affinity: as expected, the higher the affinity of the recruiter for. Ste50, the higher the output (and the quicker the ascent to a max value) They also played with the promoter strength of the gene they want to express; if they express a lot of the positive regulator, they would get more of a response (and less ste50 would produce a lower response) Termination of the egfr signal can be applied in general to most rtks: the receptor is synthesized in the.
