BIOL 202 Lecture Notes - Lecture 21: Rna Splicing, Retrotransposon, Inverted Repeat
Document Summary
Bacteria insertion elements (is elements: flanked by inverted repeat, has transposase coding region enzyme that spices at the repeats, transposase makes blunt ended cuts in donor dna and staggered cuts in target dna (6- Engineered new ty elements has galactose promotor (for control transcription) and introns (to test splicing) Mating female wild caught fruit flies to lab grown male flies all normal progeny (f1 and f2)-indicates normal in the gem line. Female lab flies and wild caught males sterile f1. Mobile element in wild genome get inserted into maternal genome. Conclusion: there must be transposon silencing mechanism in wild genome: experiment with c elegans. Transcription of pi cluster gives small rnas that degrades undesired rna product. Mobile elements outside of cluster are out of control, and can escape silencing. Crispr (or srsr) consists of repeats and spacers. Crispr and tracrrna are transcribed into rna, modified by rnase iii and cas 9 nuclease.
