BIOL 200 Lecture Notes - Lecture 20: Polyacrylamide Gel Electrophoresis, Gel Electrophoresis Of Proteins, Centrifugal Force
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Biol 200 - lecture 20, purification, detection and characterization of proteins i. The rapid spinning of a centrifuge in a machine that generates a centrifugal force that is measure in units of earth" s gravity (=1g) We have a rotor that spins with a tube full of particles (ribosome, peace of membrane, cell, proteins). Particles denser than the medium go to the bottom of the tube. Particles that are less dense than the medium, they will go the top of the tube. Particles that have the same density as the medium will not move, no matter how much g-force you put. Most of the times we"re dealing with stuff that is denser than the medium. What determines the rate at which particles move is the mass of the particles. For particles that are denser the medium, the heavier particles will move faster to the bottom of the tube.
