BIOL 112 Lecture 24: Lecture 24 - Position Cloning

• School: McGill University
• Department: Biology
• Course: BIOL 112
• Professor: Frieder Schoeck

Document Summary

Characterized by respiratory infections, digestive problems, and sterility ! Occurs in about 1/2500 caucasian newborns ! Can use cloning to determine the biochemical basis of the inherited disease ! Purpose (2) of cloning -> to understand what the gene encodes and how it works ! !- if we could gure out the gene that is mutated in cf then we could predict what protein it encodes ! missing and help us x the problem ! Knowing the sequence of the protein might help us understand what the protein does ! Knowing what the protein does would help us to understand what goes wrong when it is. Single nucleotide polymorphisms (snps): inherited variations involving a single nucleotide. There are roughly 4400 polymorphic restriction sites ! !- just as restriction enzymes (res) can cut plasmids and gene inserts, they can also cut the base (they are essentially point mutations) !