BIOC 4403 Lecture Notes - Lecture 7: Tata Box, Transfer Rna, Variable Number Tandem Repeat
23 Mar 2017
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All types of tes are represented by autonomous and non-autonomous variants. Autonomous element: encodes a complete set of enzymes characteristic of its family and is self-sufficient in terms of transposition. Non-autonomous element: transposes by borrowing the protein machinery encoded by its autonomous relatives: retrotransposons intermediate. Mrna is expressed in host cell, reverse transcribed, and cdna is integrated into host genome this is catalyzed by an rt and endonuclease/integrase (en/int), encoded by autonomous elements. 2 basic types of eukaryotic tes transposed through an rna: dna transposons: transposed by moving their genomic dna copies from one location to another, without any rna intermediate. Most tes are flanked by target site duplications (tsds) resulting from fill-in repair of staggered nicks generated at the dna target site upon insertion of tes. Four classes of retrotransposons: non-long terminal repeats (non-ltr, long-terminal repeats (ltr, penelope, dirs. Three classes of dna transposons: cut-and-paste , helitrons (rolling-circle replicative mechanism, polintons (self-synthesizing mechanism)
