HLSC 3P97 Lecture Notes - Lecture 9: Congenital Syphilis, Cardiolipin, Globus Pallidus
2 Apr 2018
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Difficult to track where disease is coming from. 3-30 years - bacteria dormant in liver and possibly spleen; non- contagious. Tests: eias, fta-abs, mha-tp (fix t. pallidum antigen to red blood cells, put serum on top, and antibodies clump on top) Cardiolipin gets released when t. pallidum damages our cells. Less sensitive than the treponemal assays due to non-specificity. Serum rpr is diluted 4 fold until we get no reaction. Higher number, more we can dilute and still get reaction, more antibody. Tppa - take gelatin and add tp antigen to them. Add serum from patient into a well, and we see that instead of gelatin spreading out, they mash together into one big particle. Tpha: take red blood cells and we attach tp antigens to each rbc in the well. Add john"s serum, and because he has antibody against t. pallidum, red cells clump together as if they"re one red cell.
