BIOL 1F90 Lecture Notes - Lecture 21: Okazaki Fragments, Primase, Hydrogen Bond
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14 Nov 2016
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Dna replication, semiconservative(each strand acts as a template to make 2 daughter strand) Many pieces of dna needs to glued back together. Three players at 1 rep fork: two forks, dna helicase extends fork unzipping two dna strands breaking h-bonds(endergonic require atp) to break bonds. Potential nucleotide enters polymerase(dexoytriphosphate) break bonds between 2 phosphate(highly exergonic) first catalyzed by h-bond if it is the complement. An enzyme called dna primase initiates dna synthesis by making a short segment of rna 10 . 12 nt long: = rna primer, will eventually be removed. Last (cid:396)i(cid:271)ose suga(cid:396) of the rna p(cid:396)i(cid:373)e(cid:396) p(cid:396)o(cid:448)ides a f(cid:396)ee 3"-oh group that can be used by dna polymerase. Rna primer must be removed and replaced with dna later. Dna pol(cid:455)(cid:373)e(cid:396)ase slides alo(cid:374)g the dna a(cid:374)d (cid:272)o(cid:448)ale(cid:374)tl(cid:455) li(cid:374)ks (cid:374)e(cid:449) (cid:374)u(cid:272)leotides to the f(cid:396)ee 3"-oh of the last nucleotide (enzyme) If a new nucleotide(proper complement) comes in it catalyzes.
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