MBLG2972 Lecture Notes - Lecture 7: Meiosis, Chromosome, Gene Mapping
5 Jul 2018
School
Department
Course
Professor
MBLG2072
Genetics and Genomics
Monica Zanuttini !
460381099
Lecture 7 Linkage and gene mapping
Recombination rates differ between males and females for most animals (e.g. male fruit flies have
no crossing over at all. !
Humans: recombination rate in males is less than females, however it looks like their genetic
distance differs. !
Molecular markers: !
•are sites of silent DNA variation not associated with any measurable phenotypic variation. !
•are critical for understanding human variation and mapping positions of disease genes. !
SNPs: Differences in DNA sequence may or may not be associated with differences in protein
structure or phenotypes. !
Detected by high resolution melt analysis (seen below), microarray, sequencing. !
!
RFLPs: When a SNP variation can destroy or create a restriction enzyme recognition sequence
(length of restriction fragments change).
Document Summary
Recombination rates di er between males and females for most animals (e. g. male fruit ies have no crossing over at all. Humans: recombination rate in males is less than females, however it looks like their genetic distance di ers. Molecular markers: are sites of silent dna variation not associated with any measurable phenotypic variation, are critical for understanding human variation and mapping positions of disease genes. Snps: di erences in dna sequence may or may not be associated with di erences in protein structure or phenotypes. Detected by high resolution melt analysis (seen below), microarray, sequencing. Rflps: when a snp variation can destroy or create a restriction enzyme recognition sequence (length of restriction fragments change). Microsatellites: number of repeats in microsatellite dna sequences are variable within the alleles of individuals. The speci c combination of alleles in a set of linked genes on a single chromosome. These alleles will tend to be passed on together during meiosis.
