BIOL1007 Lecture Notes - Lecture 3: Hydrogen Bond, Information Transfer, Electrophoresis
19 May 2018
School
Department
Course
Professor
Identify the main chemical components of nucleic acids and the interactions involved in base
pairing between nucleic acid strands. Describe how these interactions can be promoted or
disrupted in experimental situations
Tetranucleotide - contains all 4 bases
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RNA
Ribose
Screen clipping taken: 12/08/2017 9:08 PM
5 carbons
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Bases attached to
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1' (1 prime) carbon
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3' OH is end of molecule
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Prime numbering indicates something attached to carbon in SUGAR
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Sugar
phosphate
backbone
L3 - Info in biopolymers 1
Saturday, 12 August 2017
4:08 PM
Mod 1 Information transfer Page 1
Sugar
phosphate
backbone
Screen clipping taken: 26/08/2017 11:34 AM
Amine - donates
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Ketone - acceptor
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Red arrows = hydrogen bond donors, blue arrows = acceptors, differences in H bonding help identify what base it is,
different side groups and ring nitrogens
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Common, no matter what base is attached (so properties determined by backbone)
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Negative charge - each nucleotide has phosphate so negative charge
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Hydrophilic (water loving)
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Electrophoresis - need to separate strands of DNA, since it is negatively charged
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Ethanol precipitation - stripping off water around sugar phosphate backbone
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Applications
Information
face
Where the differences in bases can be seen ie. Not the sugar phosphate backbone
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Bases
Aromatic character of the 4 bases gives RNA (and DNA) its UV absorbance ie. Pi electron clouds above and below the
flat rings
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Similar UV absorbances
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Adenine, guanine, cytosine, uracil
Adenine, guanine
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Screen clipping taken: 12/08/2017 9:11 PM
Purines (double ring structure)
Cytosine, uracil
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Pyrimidines
Mod 1 Information transfer Page 2
Document Summary
Identify the main chemical components of nucleic acids and the interactions involved in base pairing between nucleic acid strands. Describe how these interactions can be promoted or disrupted in experimental situations. Prime numbering indicates something attached to carbon in sugar. Red arrows = hydrogen bond donors, blue arrows = acceptors, differences in h bonding help identify what base it is, different side groups and ring nitrogens. Common, no matter what base is attached (so properties determined by backbone) Negative charge - each nucleotide has phosphate so negative charge. Electrophoresis - need to separate strands of dna, since it is negatively charged. Ethanol precipitation - stripping off water around sugar phosphate backbone. Where the differences in bases can be seen ie. not the sugar phosphate backbone. Aromatic character of the 4 bases gives rna (and dna) its uv absorbance ie. pi electron clouds above and below the flat rings.
