483 Lecture Notes - Lecture 8: Plasmid, Digestion, Dna Gyrase

• School: University of Canberra
• Department: University of Canberra
• Course: 483
• Professor: katie cohan

Document Summary

Polymerase chain reaction (pcr: pcr is a rapid, inexpensive and simple way of copying specific dna fragments from minute quantities of source dna material, artificial process which imitates natural dna replication. Polymerase chain reaction (pcr: exponential amplification , amount of selected dna doubles every cycle (1,2,4,8,16,32,64,128 with 35 cycles, theoretically 235 = 34 billion copies) Primers: short piece of dna made in a laboratory custom built, must have some information about the sequence flanking your target, provide specificity (bind to the target sequence, tell the polymerase where to start amplifying. Acgtgccctaataataataataataataataacgtaagcta: we have performed a similar pcr experiment in the practical this week, and we will view the fragments on gels next week, forensic str analysis involves testing 13 different str regions at once. Uses of restriction enzymes: restriction enzymes are naturally found in bacteria, each restriction enzyme recognises and cuts a specific.