PHYS30001 Lecture Notes - Lecture 29: Kidney Development, Bone Morphogenetic Protein 4, Cytokeratin
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29 kidney development and its role in the programming of adult disease ii. Molecular regulation of kidney development and its role in the programming of adult disease. Whole metanephroi from rats and mice (fetuses) can be grown in vitro from e13. 5/11. 5 respectively. Allows addition of growth factors etc to media to examine effect of kidney differentiation. Glomeruli can be visualised using immunohistochemistry (wt-1 which stains podocytes or pna) Ureteric tree visualised by staining with calbindin-28 or cytokeratin. Addition of bmp-4 to culture regulates kidney development. Method: bmp-4 (260ng/ml) or vehicle added to culture and grow for 48h. Method: add dex of varying concentrations to rat metanephroi. Glucocorticoids have to be dissolved in solvent e. g. ethanol - both are inhibitory at higher dose. Allows comparison of gene expression levels at different stages of development, different sub- Allows comparison of gene expression levels at different stages of development, different sub- compartments of the kidney or from different treatment groups.
