GENE20001 Lecture Notes - Lecture 20: Chromosome, Coinfection, Bacteriophage
Document Summary
* when the two mutation are in different genes, the wt phenotype can be seen. *if plaque seen, the two mutations have complemented and therefore they are in different genes. When there is no plaque, it indicates that the mutations are in the same gene. * the experiment is set in such a way there is no significant recombination. * coinfection of host cell using a high multiplicity of infection (high moi is used so that on average each e. coli is infected by at least one of the two mutants) * coinfection creates a "diploid state" for the phage. * if the phage carry mutations in different genes, then within this cell all of the components to make new phage will be present (all proteins normally seen for wild type are present) * the progeny phage will have the same genotypes as the parents (progeny are not wild type genotype the progeny are still mutant)
