BIOL10005 Lecture Notes - Spring 2018 Lecture 11 - Polymerase chain reaction, Human genome, Denaturation (biochemistry)
20 Sep 2018
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Document Summary
Cloning: replicate a fragment of dna (placed in an organism) so that there is su cient to analyse or use in protein production = Tools to isolate and analyse region of genome. Pcr: amplify many copies of a speci c region of dna. Gel electrophoresis: separate dna fragments based on size. Tools to identify the location and amount of a gene or gene product. Rna or dna, can be applied to nucleic acids in solution, xed on surface in gel or in situ. Hybridisation: identify the location of a speci c nucleotide sequence, can be applied to. Sequencing: identify the sequence of a molecule of dna. Plasmids: vectors that carry genes in bacteria, naturally occurring, circles of dna, self. Antibiotic resistance: select for a recombinant dna molecule. Restriction enzymes: cut dna molecules at speci c sequence, ends produced are sticky replicating, multicopy or blunt, sequences are palindromes. Transformation: uptake of exogenous dna, bacteria take up external dna.
