BIOL108 Lecture Notes - Lecture 18: Polymerase Chain Reaction, Dna Profiling, Dna Sequencer

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1st and 3rd of may 2018: lecture 22 and 23 dna technology: Q1: restriction enzymes: only act on a limited range of substrates, are found in bacteriophage, recognize and cleave specific dna sequences, can be used to express recombinant proteins, are used to sequence dna, cloning and gene technology: Cloning is creating an identical copy of. Cells or organisms produced by asexual reproduction (most bacteria and single. It includes plants propagated using cuttings celled organisms) Twins are natural clones that arise when two embryos develop from a single fertilized egg: dna cloning: The manipulation of dna was made possible by the discovery and use of plasmids and restriction enzymes. Plasmids are small circular dna molecules that can be found in bacteria. They replicate independently, often researching thousands of copies per cell. Contain genes for antibiotic resistance, so that bacterial cell containing then can be selectively recovered: restriction enzymes:

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