NEUR3101 Lecture Notes - Lecture 10: Primary Cell, Ohm, Rabies Virus
Document Summary
Properties of neurons may change in culture. By slicing the brain you disconnect the other inputs. + can record from 12 hours to many months. + largely intact circuits, can combine with behaviour. Can see voltage changes without being inside the cell. Unit recording: axon running near electrode, these are the aps. Though can only see small amount of activity. Pressure squirts solution out - don"t want tip blocked. Membrane seals at the rim of the membrane, with membrane around it. The calyx of held is a giant synapse in the mammalian auditory pathway. The presynaptic (yellow) and postsynaptic (blue) side can be simultaneously patch clamped. This system allows detailed study of synaptic transmission. Whole-cell recording allows you to harvest mrna from 1 cell. Hence, a single neuron can be both genetically and electrically profiled. Full molecular profile - seal is really tight, so you don"t get contamination from other surrounding cells.
