BMSC101 Lecture Notes - Lecture 8: Scanning Electron Microscope, Electron Microscope, Optical Microscope

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17 Jun 2018

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· Scanning and Transmission electron microscopy

• Transmission Electron Microscopy (TEM):

• Measures relative difference in transparency (contrast of a

specimen to electrons

• Increases contrast by staining ultrathin tissue sections with heavy

metals (uranium)

• Images are colourless and have a resolution of 0.2 to 0.5nm

• Scanning Electron Microscopy (SEM):

• Produces images of 3D surfaces

• Electron beam is scanned across a specimen and the emission of

electrons is measured from specimens coated with a thin film of

metal (platinum)

• Colourless images

• Resolution of ~2.5nm

· Light microscope vs Electron microscope

2.2 Identify the component parts of the light microscope

2.3 Carry out care and general maintenance of light microscopes

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