BIO341H5 Study Guide - Midterm Guide: Tata-Binding Protein, Polyadenylation, Short Stature Homeobox Gene
Document Summary
Bio341 term test #1 review: dna structure & replication. Purines = adenine, guaning; 2 rings dnmps part of dna chain (not dntps) Major & minor grooves allow proteins to bind to dna. Bacterial chromosomes (circular) = 1 origin of replication (ori) Eukaryotic chromosomes = multiple origins of replication (ars) Dnab (helicase) unwinds+separate strands at 13-mer (at rich) Dna pol iii (+sliding clamp) synthesizes daughter strand. Dna pol i ( 5"-3" exonuclease & 5"-3" polymerase activity) replaces rna with dna. Dna ligase joins okazaki fragments (dna pols = dna proofreading ( 3"-5" exonuclease activity : methods of molecular genetic analysis. Pcr : 1) dna templates, 2) dna nucleotides, 3) dna pol, 4) 2 dna primers, 5) buffer a) denaturation; b) primer annealing; c) primer extension. Vntrs : genetic marker; forensic genetic analysis; codominant hereditary pattern. Sanger sequencing (dideoxy) : abundant dntps and little ddntp; chain termination.
