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You are working for a biotech company that has designed a new enzyme that modifies the headgroups of phosphorylated PI lipids in vitro. This enzyme is extremely active and soluble, however it has poor affinity for a biological membrane. Your job is to modify the enzyme to increase its affinity for the membrane.
1. How you would alter the enzyme to increase its affinity for a biological membrane? Explain your choices.
2. What experiments would you perform to test your designed protein?
3. What would be the expected outcome of these experiments be if you were successful in your task?

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