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2 Nov 2021
Background and context
Fred Sanger invented the chain termination method of sequencing. This approach involves utilising a primer and a regular deoxynucleotide to replicate a single-stranded DNA template (dNTP). Labeled dideoxynucleotides are combined with the primer and dNTP (ddNTPs). Because the ddNTP lacks a 
' – OH group, it functions as a chain terminator, preventing more nucleotides from being added to the new chain.
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