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13 Dec 2019

Preparing Buffers!

Molecular weights:

NaH2PO4: 119.96 g/mol

Na2HPO4: 141.96 g/mol

Tris base: 121.14 g/mol

Tris-HCl: 157.60 g/mol

Sodium acetate: 58.04 g/mol

pKa:

Tris: 8.30

Phosphate: 7.21

Acetate: 4.76

1) Prepare 750 mL of 0.5 M phosphate buffer, pH 6.7. You have available both NaH2PO4 and Na2HPO4.

2) Prepare 750 mL of 0.5 M phosphate buffer, pH 7.8. You have available Na2HPO4, 6 M NaOH and 6 M HCl.

3) Prepare 750 mL of 0.5 M phosphate buffer, pH 7.2. You have available NaH2PO4, 6 M NaOH and 6 M HCl.

4) Prepare 1.5 L of 300 mM Tris buffer, pH 8.2. You have available both Tris base and Tris-HCl.

5) Prepare 1.5 L of 300 mM Tris buffer, pH 7.8. You have available Tris base, 6 M NaOH and 6 M HCl.

6) Prepare 1.5 L of 300 mM Tris buffer, pH 8.8. You have available Tris-HCl, 6 M NaOH and 6 M HCl.

7) Prepare 500 mL of 3.0 M acetate buffer, pH 5.0. You have available both glacial acetic acid and sodium acetate (note: glacial acetic acid is 17.5 M).

8) Prepare 1L of 50 mM phosphate buffer at pH 7.50 using only Na2HPO4 and 1M HCl.

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Bunny Greenfelder
Bunny GreenfelderLv2
17 Dec 2019
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