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Casey Durgan
Casey DurganLv2
4 Aug 2019
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Related questions

If an enzyme reaction rate is approaching the Vmax, with a large excess of substrate present, which of the following would have the least effect on the rate of reaction?

Add moderate amount of a competitive inhibitor
Add a moderate amount of a non-competitive inhibitor
Add more enzyme to the mixture

Add a moderate amount of an irreversible inhibitor

Decrease the Temperature of the Reaction by a moderate amount

How can Vmax be approached despite the presence of a competitive inhibitor?

Increase enzyme concentration to very high levels
Increase substrate concentration to very high levels
Increase the temperature of the reaction
Decrease the temperature of the reaction
Add a non-competitive inhibitor to the reaction mixture

6. (10 points) When double-stranded DNA is heated, the two strands separate into single strands in a process called melting or denaturation. The temperature at which half of the duplex DNA molecules are intact and half have melted is defined as the Tm.

a. Do you think Tm is a constant, or can it depend on other small molecules in the solution? Do you think high salt concentrations increase, decrease, or have no effect on Tm

b. Under standard conditions, the expected melting temperature in degrees Celsius can be calculated from the equation Tm = 59.9 + [0.41 รƒย— %(G + C)] รขย€ย“ (675/length of duplex). Does the Tm increase or decrease if there are more G + C (and thus fewer A + T) base pairs? Does the Tm increase or decrease as the length of DNA increases? Why?

c. Calculate the predicted Tm for a stretch of double helix that is 100 nucleotides long and contains 50% G + C content.

When double-stranded DNA is heated, the two strands separate into single strands in a process called melting or denaturation. The temperature at which half of the duplex DNA molecules are intact and half have melted is defined as the Tm.

A. Do you think Tm is a constant, or can it depend on other small molecules in the solution? Do you think high salt concentrations increase, decrease, or have no effect on Tm?

B. Under standard conditions, the expected melting temperature in degrees Celsius can be calculated from the equation Tm = 59.9 + [0.41 รƒย— %(G + C)] รขย€ย“ (675/length of duplex). Does the Tm increase or decrease if there are more G + C (and thus fewer A + T) base pairs? Does the Tm increase or decrease as the length of DNA increases? Why?

C. Calculate the predicted Tm for a stretch of double helix that is 100 nucleotides long and contains 50% G + C content.

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