Your teacher decides to give you some frog DNA to digest with these restriction enzymes. Thinking about structure of the frog DNA, provide 2 reasons why digesting frog DNA may not work or may have abnormal or incomplete digests.
I know that methylation of DNA is one but I cannot find the other reason.
Your teacher decides to give you some frog DNA to digest with these restriction enzymes. Thinking about structure of the frog DNA, provide 2 reasons why digesting frog DNA may not work or may have abnormal or incomplete digests.
I know that methylation of DNA is one but I cannot find the other reason.
For unlimited access to Homework Help, a Homework+ subscription is required.
Related textbook solutions
Related questions
View/perform/read ALL THREE of the following prior to answeringthe questions.
http://highered.mcgraw-hill.com/olcweb/cgi/pluginpop.cgi?it=swf::535::535::/sites/dl/free/0072437316/120078/micro10.swf::Stepsin Cloning a Gene (Links to an external site.)
http://www.discoverbiotech.com/wiki/-/wiki/Main/Applications ofCloning (Links to an external site.)
http://www.wiley.com/college/boyer/0470003790/animations/cloning/cloning.htm(Links to an external site.)
Skip to question text.
From the list below, which of the following is the most logicalsequence of steps for splicing foreign DNA into a plasmid andinserting the plasmid into a bacterium?
I. Transformbacteria with recombinant DNA molecule
II. Cutthe plasmid DNA using restriction enzymes
III. Extractplasmid DNA from bacterial cells
IV. Hydrogen-bondthe plasmid DNA to nonplasmid DNA fragments
V. Useligase to seal plasmid DNA to nonplasmid DNA
IV, V, I, II, III |
III, II, IV, V, I |
III, IV, V, I, II |
II, III, V, IV, I |
I, II, IV, III, V |
Flag this Question
Plasmids (or vectors) are important in biotechnology becausethey are
a vehicle for the insertion ofrecombinant DNA into bacteria. |
surfaces for respiratoryprocesses in bacteria. |
recognition sites onrecombinant DNA strands. |
surfaces for protein synthesisin eukaryotic recombinants. |
proviruses incorporated intothe host DNA |
Flag this Question
Plasmids are put into bacterial cells by
restriction enzymes |
DNA ligase |
binding of cohesive stickyends |
transformation |
Flag this Question
Restriction enzymes usually
cut donor DNA evenly so smoothedges result |
cut donor DNA but do notaffect plasmids |
make staggered cuts atspecific sequences in DNA in both donor DNA and plasmid |
are used in ligating plasmidsinto bacterial host cells |
more than one of theabove |
Flag this Question
After combining DNA fragments in a cloning experiment, ___ isused to covalently join the DNA segments.
Restriction enzyme |
DNA Ligase |
Reverse transcriptase |
DNA polymerase |
Helicase |
Flag this Question
It is theoretically possible for a gene from any organism tofunction in any other organism. Why is this possible?
All organisms haveribosomes. |
All organisms have the samegenetic code. |
All organisms are made up ofcells. |
All organisms have similarnuclei. |
All organisms have transferRNA. |
Flag this Question
Skip to question text.
Assume that you are trying to insert a gene into a plasmid andsomeone gives you a DNA sample cut with restriction enzyme X. Thegene you wish to insert from the given sample has sites on bothends for cutting by restriction enzyme Y. You have a plasmid with asingle site for Y, but not for X. Your strategy should be to
cut the plasmid withrestriction enzyme X and insert the fragments cut with Y into theplasmid. |
cut the plasmid with enzyme Xand then insert the gene into the plasmid. |
cut the DNA again withrestriction enzyme Y and insert these fragments into the plasmidcut with the same enzyme. |
cut the plasmid twice withrestriction enzyme Y and ligate the two fragments onto the ends ofthe human DNA fragments cut with restriction enzyme X. |
insert the fragments cut withX directly into the plasmid without cutting the plasmid. |
Flag this Question
Which of the following is/are false in regard to expressionplasmids (also called expression vectors)?
They are used to make proteinsusing a cloned gene. |
They contain a promotor. |
They are the first plasmidtype used to clone a gene. |
They contain aterminator. |
More than one of the above isfalse. |
Flag this Question
What is NOT a potential problem(s) associated with usingbacteria containing a cloned eukaryotic gene (e.g. a human gene) toproduce a functional protein?
If the eukaryotic genecontains introns the bacteria will not remove them and theresulting amino acid sequence will be different that that made by aeukaryote. |
The bacteria may not fold theprotein correctly. |
The bacteria may degrade theprotein. |
All of the above are potentialproblems. |
Flag this Question
Cloning allows for production of proteins in much larger amountsthan occurs in the cells from which the gene is isolated.
True |
False |
Flag this Question
Question 111 pts
Gene cloning is used to do all of the following except
Make insulin |
Making genetically identicalanimals (e.g. Dolly the sheep) |
Make vaccines |
Perform Gene Therapy |
Making genetically engineeredplants |
Flag this Question
In your
1. | In addition to identifying the genetic material, the experiments of Avery, MacLeod, and McCarty with different strains of Streptococcus pneumoniae demonstrated that | ||||||||||
|
2. | In order to show that DNA in cell extracts is responsible for genetic transformation in Streptococcus pneumoniae, important corroborating evidence should indicate that _______ also destroy transforming activity. | ||||||||||
|
3. | Based on what you have learned about the experiments conducted by Griffith and Avery and colleagues with bacteria, which of the following would result in transformation of living R cells? | ||||||||||
|
4. | A-T base pairs in a DNA double helix | ||||||||||
|
5. | If 23 percent of the bases in a sample of double-stranded DNA are adenine, what percentage of the bases are uracil? | ||||||||||
|
6. | The uniform diameter of the DNA structure provides evidence for | ||||||||||
|
7. | If a sequence of one strand of DNA is 5â²-TGACTATC-3â², what is the complementary strand? | ||||||||||
|
8. | What structural aspect of the DNA facilitates dissociation of the two DNA strands for replication? | ||||||||||
|
9. | If the MeselsonâStahl density gradient experiment had resulted in two bands of DNA molecules after only one round of replication, one containing only 15N and the second only 14N, this result would have indicated that replication was | ||||||||||
|
10. | The nucleoside analogue acyclovir, which is used to treat herpes simplex virus (HSV) infections, lacks a 3â² hydroxyl group (âOH). Predict what will happen if the host cell DNA polymerase incorporates a molecule of acyclovir into an elongating strand of HSV DNA. | ||||||||||
|
11. | Which of the following does not demonstrate the stability of the DNA double helix? | ||||||||||
|
12. | What effect would a primase inhibitor have on DNA replication? | ||||||||||
|
13. | To replicate their DNA in a timely manner, most eukaryotic chromosomes | ||||||||||
|
14. | Which statement about DNA replication is false? | ||||||||||
|
15. | In many eukaryotes, there are repetitive sequences called telomeres at the ends of chromosomes. After successive rounds of DNA replication, the _______ strand becomes shorter. In some cells, an enzyme called _______ repairs the shortened strand. | ||||||||||
|
16. | A researcher studies normal human fibroblast cells. They can be maintained in culture but die off after about 30 cell generations. Unexpectedly, a colony of cells continues to survive and divide past 30 generations. Which scenario is most likely true for these cells? | ||||||||||
|
17. | If DNA polymerase III introduces an incorrect nucleotide, what is the first corrective action made by the DNA repair system? | ||||||||||
|
18. | Choose the correct order of the following four events in the excision repair of DNA: (1) Base-paired DNA is made complementary to the template. (2) Damaged bases are recognized. (3) DNA ligase seals the new strand to existing DNA. (4) Part of a single strand is excised. | ||||||||||
|
19. | Six complete cycles of PCR should result in a _______-fold increase in the amount of DNA. | ||||||||||
|
20. | When double-stranded DNA is heated to temperatures above 90°C, it denatures. Denaturation is a process that | ||||||||||
|