Question

E. coli cells are growing in medium containing lactose but no glucose. Briefly describe the consequence of the following changes (one to two sentences for each condition).

a. Addition of high concentration of glucose.

b. A mutation that inactivates galactoside permease.

c. A mutation that inactivates beta-galactosidase.

d. A mutation that affects binding of CAP to c-AMP

e. A mutation that affects binding of inducer to LacI

f. A lac operator mutation that deletes all of the O1