Biology And Biomedical Sciences BIOL 2960 Lecture 38: Sp18 L37-38 Guide

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At the end of this unit, you should be able to define and/or use the following terms and concepts: genomics: Functional genomics: microarray: cdna probe attached to substrate, labelled cdna copy of mrna from different cell samples. Purpose: quantitatively compare mrnas expressed in two samples: chromatin immunoprecipitation followed by sequencing (chip-seq): fix dna to bound proteins, dna fragmentation, antibody immunoprecipitation, next generation sequencing. Purpose: determine where within the genome a protein is physically located (i. e. to what sequence a. Dna binding protein associates) grna or sgrna), cas9 nuclease, double-stranded break, targeted by. Rna/dna complementarity: outcome of breaks: (1) random mutations by error-prone non- homologous end joining (nhej) or (2) specific mutations with homologous recombination using donor dna. Purpose: introduce a mutation at specific sequence in the genome: an approach for genome sequencing: Whole genome shotgun (wgs): random fragmentation, addition of adaptors, computer assembly using sequence overlaps, illumina sequencing.

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