CHEM123L Lecture Notes - Lecture 5: Iodine Test, Biuret Test, Reducing Sugar

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In this experiment, 12 samples of substances will be tested to see what macromolecules they represent. There are four main macromolecules which are carbohydrates, lipids, proteins and nucleic acid. They are important to the cell as they have many important functions, for example, they aid in the transportation of nutrient, support, storing information (alberts et al,2015). In this experiment 3 of the macromolecules will be studied which are carbohydrates and proteins using 3 tests. The three tests are, iodine test for starch and glycogen, Benedict"s test for reducing sugars and biuret test for proteins (alberts et al, 2015). Iodine test for starch and glycogen involves a color change to black/blue in the presence of starch and a color change to reddish-brown in the presence of glycogen. The 12 solutions that will be tested are 1% glucose, 0. 3% glucose-1-phosphate, 1% maltose, honey, 1% sucrose, 1% lactose, 1% glycogen, 1% starch, protein, beer, distilled water, and an unknown solution labelled as sample.