BIOL207 Lecture Notes - Lecture 2: Nucleic Acid Double Helix, Dna Replication, Semiconservative Replication
Document Summary
Required readings and problems: open genetics lectures chapter 2. 9th ed. pp 275-291, problems: chapter 7: 1-13, 15-25, 28. Double helix structure provides a chemical explanation for the transmission of genes (transmission genetics) Information flows from nucleic acid to protein (one-direction only) Examine one prediction of watson and crick double helix model: that of semi-conservative replication (not conservative or dispersive, each daughter duplex contains one parental and one newly synthesized strands. 1st generation single band between 15n and 14n controls. 2nd generation two bands (1 between 15n and 14n controls, 1 at 14n control) Semi-conservative: 1st generation 1 band, 2nd generation 2 bands (correct) Conclusion: evidence that dna replication is semi-conservative refute conservative and dispersive models, supports watson and crick double helix model. Dna synthesis well described in e. coli less known about eukaryotes. Dna pol (cid:272)o(cid:374)ti(cid:374)uous s(cid:455)(cid:374)thesis o(cid:374) 3" e(cid:374)d initially started with rna primer: lagging strand. Must be synthesized in short discontinuous segments.