BIOL 2303 Lecture Notes - Lecture 14: Primary And Secondary Antibodies, Antibody, Antigen
Document Summary
Dna gives us proteins (that"s it, it"s mostly proteins that gives phenotypes and morphology) morphology of the individual. Proteins gives us different phenotypes which determines the. Nucleic acids for differentiation for different microorganisms. If we get a protein, inject it in a mouse, the mouse immune system will create antibodies for fight antigen, and this will be primary antibody to fight (ex: salmonella) The isotope (in cases of radioactivity) that is used for detection of protein is s^35. It"s the isotope that gets incorporated into amino acids. Dna made up of nucleotides, agct (building blocks of dna) Dna has 2 ends, 5" end and 3" end. How can we recognize the dna of interest, (ex. Ans: we can use things like a specific piece of dna called prob (or primer) , which is specific to a region of salmonella, prob can only bind to a specific sequence which is unique to salmonella.